B21, China Town Mall, Midrand
Specification
| Model | Q2000A | Q2000B | Q2000C |
| Instrument Performance | |||
| Sample Block Capacity | 96 wells * 0.1 ml | ||
| Reaction Volume | 10-50ul | ||
| Tubes Option | White or clear PCR tubes or strips or 96 well PCR plate, with optical flatcap | ||
| Heating & Cooling Technology | New generation Peltier technology alow 100.000 cycles | ||
| Control Methods | Operated via PC or self.contained touch screen on instrument | ||
| Language | English | ||
| Communications | USB2.0 or LAN | ||
| Display | 10" Color TFTLCD and Touch Screen | ||
| Max. Number of Programs | Max.15,000 programs onboard, unlimited storage of protocols with USB flash drive | ||
| Temperature | |||
| Block Temp.Range | 0°C-105°C | ||
| Max. Heating Rate | 6°C/sec | ||
| Max. Cooling Rate | 5*C/sec | ||
| Temp.Uniformity | ≤± 0.2°C (at90°C) | ||
| Temp.Accuracy | ≤± 0.1°C (at 90°C) | ||
| Display Resolution | 0.1°C | ||
| Heat Lid Temp. Range | 30°C-112°C | ||
| Temp.Control Mode | Block & Calculated sample | ||
| Gradient Range | 30°C ~ 100°C | ||
| Temp.Differential Range | 1°C ~ 30°C | ||
| Fluorescence Detection | |||
| Excitation | Long life LED lamps | ||
| Detection | CCDs | ||
| Dynamic Range | 1-10^10 | ||
| Senstivity | ≥1copy | ||
| Calibrated Dyes at Installation | F1: FAM, SYBR Green F2: VIC, HEX, JOE, Cy3, NED | F1: FAM, SYBR Green F2: VIC, HEX, JOE, Cy3, NED F3: ROX, TEXAS-RED F4: CY5 | F1: FAM, SYBR Green F2: VIC, HEX, JOE, Cy3, NED F3: ROX, TEXAS-RED F4: CY5 F5: CY5.5 F6: Reserved |
| Fluorescence Excitation Range | 300-800m | ||
| Fluorescence Detection Range | 500-800nm | ||
| Data Export Formats | Excel, TXT | ||
| Other Features | |||
| AC Power Supply | 100~ 240V, 50~ 60Hz | ||
| Consumption | 600W | ||
| Net Weight | 13KG | ||
| Dimension (L*W*H) | 334 * 280* 365 mm | ||
| Computer Operating systems | Windows 10, Windows, Windows XP |
Main Advantages of Real Time PCR System:
1. Imported Semiconductor Chip: Top brand Peltier elements from Marlow(U.S.A)adopted, ensure long life of 1000,000 cycles and fast
ramping rate up to 6°C/s.
2. T-opticalm Technology: T-opticalIM technology, reduce background noise, improve fluorescence signal sensitivity and signal to
noise ratio.
3. Articulating Display Screen: The angle of display could be adjusted to the best view.
4. Synchronous Detection: Simultaneous detection of wells, not in sequence.
5. Self-contained Touch Screen: Users could view qPCR process and run PCR protocol through self-contained 10"TFT LCD and touch
screen.
6. Patented Optical Design: Innovative SSLPT CCD imaging technology for qPCR, avoiding more moving parts problems like overheat,
wear and off-center. Not optical fiber based, avoiding break and block by dust.
7. Long Lifeled: Long life LED lamps to excite fluorescence, no need for replacement in the future.
8. Gradient Function: Sample wells with temperature gradient function, convenient to optimize PCR conditions.
Remote Control: Equipped with LongGene's "Optimum qPCR Design & Analysis Software" for remote operation of instruments and
analysis of results.
9. Drawer Design: The drawer design of sample block, makes it easier to pick and place PCR tubes and plates.
10. Lifetime Free for Software Upgraded: LongGene's "Optimum qPCR Design & Analysis Software" could be upgraded for free.
working principle:1. In the lysis buffer, disrupt the cells and release the nucleic acid into the buffer.2. Add magnetic beads to the lysis buffer and mix thoroughly to make the nucleic acid adsorb to the surface of the magnetic beads.3. Repeatedly clean the surface of the magnetic beads to remove impurities such as proteins or salts that were originally entrained.4. Transfer the magnetic beads to the elution buffer and mix well. Nucleic acid is from the surface of magnetic beadsFall off and dissolve into the elution buffer.5. Remove the magnetic beads from the buffer and recover the nucleic acid solution.
